I got married in L.A. in 2013 me amd my wife have been seperated since 2017. I moved to vegas in 2018 and been here ever since. Can i file in Nevada?

Short answer:  yes.  Long answer: it depends on what you wish to accomplish; it is also unclear who lives where, at least as to the children.  The Nevada court can dissolve the marriage, but it is clear that there are other issues, including child custody and support.  Look at the materials posted here and here, and probably check out the pages on Divorce, Property, and Spousal Support, and then especially the article "The Basics of Family law Jurisdiction" (on both the Child Support and Child Custody pages), and then call, ask any preliminary follow up questions, and consider scheduling a consultation to go over all of your facts circumstances, rights, obligations, and options.