If my spouse took money out of our joint accounts and gave the funds to her sisters so that I do not have any access to it, can i legally pursue this?

Yes, you can pursue this money in a divorce.  You may have to obtain documents from your wife or her banks that show where they funds have gone, but assuming you can trace it back to a marital account, then it should be considered marital property.  If the money is out of her control, however, because it is in the name of a third party, the court may find that she has "wasted" this marital asset and she could be ordered to pay you back your share or it would be deducted from any other property or assets she would otherwise be awarded in equitable distribution.  Be sure to keep any bank statements or other documents that show this money being withdrawn. This answer is given in accordance with the laws of Virginia and may not be applicable in any other state.  It should not be construed as legal advice, as that would require a more thorough analysis of all of the facts involved in a specific case.