My daughter is about to be in a custody disagreement with the father of her twins. Can an ad litem be involved before court has begun?

If the child was born out of wedlock, is paternity established? Under Wisconsin law, the (alleged) father would have no legal rights to the child under paternity is established and court orders are set on custody and placement. I realize that doesn't specifically answer your question. The answer to your question is generally no. The court will first order the parties to mediate any disputed custody and placement issues and only if mediation is unsuccesful will appoint a guardian ad litem for the twins. While there may be some exceptions to doing it that way, that is the usual course of aciton.