We lived in California for 33 years, separated Aug 2013. I moved to Nevada Mar 2014. Can I now file for divorce in Nevada?

Well, the short answer is "yes, you can," but you may well not want to. The problem is that unless your estranged spouse voluntarily agrees to participate in the Nevada action, the NV courts will have jurisdiction only over "status" and what little property is located here -- not over the CA real estate, your soon-to-be-ex, issues of support, or several other matters.  You might want to start with the article “The Basics of Family Law Jurisdiction,” posted at http://willicklawgroup.com/published-works/.  There is a lot more on the web site that might be of interest to you as well.  Whether, tactically, it makes sense to litigate the divorce in Nevada, and other issues in California, is a matter best gone over, in detail, in consultation with a family law specialist well versed in matters of jurisdiction.